ស្វែងរកតាមប្រភេទឯកសារ
ស្វែងរកតាមប្រភេទដំណាំ
អត្ថបទថ្មីៗ
ចំនួនភ្ញៀវចូលទស្សនា
        
ថ្ងៃនេះ
ថ្ងៃម្សិលមិញ
សប្តាហ៍នេះ
សប្តាហ័មុន
ខែនេះ
ខែមុន
ពាក្យគន្លឺះ  

ស្វែងរក
ស្វែងរកលំអិត
បណ្ណាល័យ
ចំណងជើង:
Resistance to Sri Lankan Cassava MosaicVirus (SLCMV) in Genetically Engineered Cassava cv. KU50 through RNA Silencing, by Valentine Otang Ntui, Kynet Kong, Raham Sher Khan, Tomoko Igawa, Gnanaguru Janaky Janavi , Ramalingam Rabindran, Ikuo Nakamura , Masahiro Mii, PLOS ONE | DOI:10.1371/journal.pone.0120551 April 22, 2015
បរិយាយ:
Abstract
Cassava ranks fifth among the starch producing crops of the world, its annual bioethanol yield is higher than for any other crop. Cassava cultivar KU50, the most widely grown culti- var for non-food purposes is susceptible to Sri Lankan cassava mosaic virus (SLCMV). The objective of this work was to engineer resistance to SLCMV by RNA interference (RNAi) in order to increase biomass yield, an important aspect for bioethanol production. Here, we produced transgenic KU50 lines expressing dsRNA homologous to the region between the AV2 and AV1 of DNA A of SLCMV. High level expression of dsRNA of SLCMV did not in- duce any growth abnormality in the transgenic plants. Transgenic lines displayed high lev- els of resistance to SLCMV compared to the wild-type plants and no virus load could be detected in uninoculated new leaves of the infected resistant lines after PCR amplification and RT-PCR analysis. The agronomic performance of the transgenic lines was unimpaired after inoculation with the virus as the plants presented similar growth when compared to the mock inoculated control plants and revealed no apparent reduction in the amount and weight of tubers produced. We show that the resistance is correlated with post-transcription- al gene silencing because of the production of transgene specific siRNA. The results dem- onstrate that transgenic lines exhibited high levels of resistance to SLCMV. This resistance coupled with the desirable yield components in the transgenic lines makes them better can- didates for exploitation in the production of biomass as well as bioethanol.

Full Article
 
keyword:
N/A
Abstract
Cassava ranks fifth among the starch producing crops of the world, its annual bioethanol yield is higher than for any other crop. Cassava cultivar KU50, the most widely grown culti- var for non-food purposes is susceptible to Sri Lankan cassava mosaic virus (SLCMV). The objective of this work was to engineer resistance to SLCMV by RNA interference (RNAi) in order to increase biomass yield, an important aspect for bioethanol production. Here, we produced transgenic KU50 lines expressing dsRNA homologous to the region between the AV2 and AV1 of DNA A of SLCMV. High level expression of dsRNA of SLCMV did not in- duce any growth abnormality in the transgenic plants. Transgenic lines displayed high lev- els of resistance to SLCMV compared to the wild-type plants and no virus load could be detected in uninoculated new leaves of the infected resistant lines after PCR amplification and RT-PCR analysis. The agronomic performance of the transgenic lines was unimpaired after inoculation with the virus as the plants presented similar growth when compared to the mock inoculated control plants and revealed no apparent reduction in the amount and weight of tubers produced. We show that the resistance is correlated with post-transcription- al gene silencing because of the production of transgene specific siRNA. The results dem- onstrate that transgenic lines exhibited high levels of resistance to SLCMV. This resistance coupled with the desirable yield components in the transgenic lines makes them better can- didates for exploitation in the production of biomass as well as bioethanol.

Full Article
 
ចំណងជើង:
Transgenic Tobacco Lines Expressing Defective CMV Replicase-Derived dsRNA Are Resistant to CMV-O and CMV-Y, by Valentine Otang Ntui, Kong Kynet, Raham Sher Khan, Mari Ohara, Yasuko Goto, Manabu Watanabe, Masanobu Fukami, Ikuo Nakamura, Masahiro Mii. Mol Biotechnol DOI 10.1007/s12033-013-9681-5
បរិយាយ:

Abstract

Cucumber mosaic virus (CMV) is a tripartite, positive sense RNA virus causing infections and yield losses to many plant species. Here, we generated a construct containing inverted repeat of 1,793 bp fragment of defective CMV replicase gene derived from RNA2 of cucumber mosaic virus strain O (CMV-O). The replicase gene was modified by deleting a 9 bp region between nucleotides 1909–1918. This caused a deletion in the active centre motif of polymerases, producing defective translated product 9 nucleotides shorter than the full length protein. The RNAi construct containing inverted repeat of the defective gene was used to produce transgenic tobacco lines expressing CMV-derived double-stranded RNA via Agrobacterium-mediated transformation. Of the four transgenic lines inoculated with CMV-O or CMV-Y in vitro and ex vivo, three lines (T1, T4 and T5) showed immunity to both strains of CMV as no symptoms were detected, whereas one line (T7) exhibited high resistance with mild symptoms limited to inoculation portions. No virus could be detected in uninoculated new leaves of the transgenic lines after RT-PCR and Dot-immunobinding assay analyses. Small interfering RNAs present in transgenic lines before and after virus challenge indicates that the resistance was acquired through RNA silencing.

Full article
 

keyword:
CMV dsRNA PTGS Replicase gene RNAi

Abstract

Cucumber mosaic virus (CMV) is a tripartite, positive sense RNA virus causing infections and yield losses to many plant species. Here, we generated a construct containing inverted repeat of 1,793 bp fragment of defective CMV replicase gene derived from RNA2 of cucumber mosaic virus strain O (CMV-O). The replicase gene was modified by deleting a 9 bp region between nucleotides 1909–1918. This caused a deletion in the active centre motif of polymerases, producing defective translated product 9 nucleotides shorter than the full length protein. The RNAi construct containing inverted repeat of the defective gene was used to produce transgenic tobacco lines expressing CMV-derived double-stranded RNA via Agrobacterium-mediated transformation. Of the four transgenic lines inoculated with CMV-O or CMV-Y in vitro and ex vivo, three lines (T1, T4 and T5) showed immunity to both strains of CMV as no symptoms were detected, whereas one line (T7) exhibited high resistance with mild symptoms limited to inoculation portions. No virus could be detected in uninoculated new leaves of the transgenic lines after RT-PCR and Dot-immunobinding assay analyses. Small interfering RNAs present in transgenic lines before and after virus challenge indicates that the resistance was acquired through RNA silencing.

Full article
 

ចំណងជើង:
Nutrient balances for cassava cultivation in Kampong Cham province in Northeast Cambodia, by Sopheap, U Patanothai, A Aye, Tin Maung , U. Sopheap et al. / International Journal of Plant Production (2012) 6(1): 37-58
បរិយាយ:
Abstract
In Cambodia, cassava is mostly grown with little or no fertilizer inputs, but the magnitudes of nutrient balances are not known. This study was conducted to assess nutrient balances for cassava cultivation in Kampong Cham province in Northeast Cambodia. Forty-five households in four cassava production zones were interviewed in relation to their cultural practices and crop residue management, upon which sources of nutrient inputs and outputs were based. Chemical fertilizer, manure, planting materials and rainfall were the defined inputs, while cassava roots and stumps were the outputs. Crop cutting was undertaken in the cassava fields of the 45 households to obtain weights of roots and other plant parts. Nutrient balances were calculated for the individual fields based on nutrient contents of the component sources obtained from the literatures. The results showed negative balances for all the nutrients evaluated. The imbalances were most serious for N, K and Ca with the averages of -64.45 kg N, -52.83 kg K and -10.83 kg Ca ha-1, but were less serious for P and Mg with the averages of -2.85 kg P and -7.20 kg Mg ha-1. These negative balances were the consequence of low nutrient inputs in current practices where only a few farmers applied low rates of chemical fertilizer or manure. Continued use of current practices will threaten the sustainability of cassava production in Cambodia. For long term productivity of the crop, the application of organic manures together with lime and chemical fertilizers high in N and K is recommended.

Full Article
keyword:
Soil nutrients; Agricultural sustainability; Soil fertility management; Cassava fertilization; Long-term productivity.
Abstract
In Cambodia, cassava is mostly grown with little or no fertilizer inputs, but the magnitudes of nutrient balances are not known. This study was conducted to assess nutrient balances for cassava cultivation in Kampong Cham province in Northeast Cambodia. Forty-five households in four cassava production zones were interviewed in relation to their cultural practices and crop residue management, upon which sources of nutrient inputs and outputs were based. Chemical fertilizer, manure, planting materials and rainfall were the defined inputs, while cassava roots and stumps were the outputs. Crop cutting was undertaken in the cassava fields of the 45 households to obtain weights of roots and other plant parts. Nutrient balances were calculated for the individual fields based on nutrient contents of the component sources obtained from the literatures. The results showed negative balances for all the nutrients evaluated. The imbalances were most serious for N, K and Ca with the averages of -64.45 kg N, -52.83 kg K and -10.83 kg Ca ha-1, but were less serious for P and Mg with the averages of -2.85 kg P and -7.20 kg Mg ha-1. These negative balances were the consequence of low nutrient inputs in current practices where only a few farmers applied low rates of chemical fertilizer or manure. Continued use of current practices will threaten the sustainability of cassava production in Cambodia. For long term productivity of the crop, the application of organic manures together with lime and chemical fertilizers high in N and K is recommended.

Full Article


ចំណងជើង:
Rice Germplasm Catalog of Cambodia IV
បរិយាយ:
 
keyword:
N/A
 


ចំណងជើង:
សមិទ្ធផលនៃការស្រាវជ្រាវនិងអភិវឌ្ឃន៍បច្ចេកវិទ្យា (1999-2017)
បរិយាយ:
 
keyword:
N/A
 


ចំណងជើង:
ស្មៅចង្រៃនៃដំណាំស្រូវ នៅអាស៊ី
បរិយាយ:

ការរុករានពីស្មៅចង្រៃមកលើផលដំណាំគឺជាការព្រួយបារម្ភរបស់កសិករគ្រប់ៗរូប។​ ដោយភាពស្រដៀងគ្នានៃ​ប្រព័ន្ធផលិត​កម្ម​ដំណាំ​ស្រូវ ជារឿយៗ​កសិករ​នៅ​តំបន់​អាស៊ី​តែង​តែ​ប្រ​ឈម​ជា​មួយ​ប្រភេទ​ស្មៅ​ចង្រៃ​តូចៗ​ដដែលៗ​ឬ​ស្រដៀង​ៗ​​គ្នា។​​​ ពូជ​ស្មៅ​ចង្រៃ​ទាំង​នេះ​មាន​ទំហំ​តូច​ក៏​ពិត​មែន ប៉ុន្តែ​មាន​ឥទ្ធិពល​ខ្លាំង​នៅ​ពេល​វា​ដុះ​រួម​គ្នា​ជា​ច្រើន ដែល​បង្ក​ឲ្យ​ក្លាយ​ជា​ពូជ​ស្មៅ​អាក្រក់​ជាង​គេ​បំផុត​លើ​ពិភពលោក​ជា​ច្រើន​ផង​ដែរ។ នៅ​ក្នុង​សៀវភៅ​មគ្គទេសក៍​នេះ យើង​បាន​ព្យាយាម​ប្រមូល​ព័ត៌មាន​ជាក់​ស្តែង​អំពី​ប្រភេទ​ស្មៅ​ចង្រៃ​នៅ​ក្នុង​ស្រែ​ទូទៅ​មួយ​ចំនួន​នៅ​តំបន់​អាស៊ី រួម​ទាំង​ព័ត៌មាន​អំពី​រុក្ខវិទ្យា បរិស្ថាន​វិទ្យា ភាព​ធន់​ទ្រាំ​ទៅ​នឹង​ថ្នាំ​សម្លាប់​ស្មៅ​និង​ការ​កម្ចាត់​តាម​វិធីសាស្រ្ត​ដាំដុះ ជា​លក្ខណៈ​អត្ថបទ​ខ្លីៗ​ដែល​អាច​ផ្តល់​ភាព​ងាយ​ស្រួល​ក្នុង​ការ​ប្រើ​ប្រាស់។​ លើស​ពីនេះ​ទៅ​ទៀត យើង​ក៏​បាន​ដាក់​បញ្ចូល​ជា​រូបភាព​ដើម្បី​ឲ្យ​យល់​កាន់​តែ​ច្បាស់​និង​បាន​ត្រឹម​ត្រូវ​អំពី​អត្តសញ្ញាណ​នៃ​ស្មៅ​ចង្រៃ​នីមួយៗ​ផងដែរ។

keyword:
N/A

ការរុករានពីស្មៅចង្រៃមកលើផលដំណាំគឺជាការព្រួយបារម្ភរបស់កសិករគ្រប់ៗរូប។​ ដោយភាពស្រដៀងគ្នានៃ​ប្រព័ន្ធផលិត​កម្ម​ដំណាំ​ស្រូវ ជារឿយៗ​កសិករ​នៅ​តំបន់​អាស៊ី​តែង​តែ​ប្រ​ឈម​ជា​មួយ​ប្រភេទ​ស្មៅ​ចង្រៃ​តូចៗ​ដដែលៗ​ឬ​ស្រដៀង​ៗ​​គ្នា។​​​ ពូជ​ស្មៅ​ចង្រៃ​ទាំង​នេះ​មាន​ទំហំ​តូច​ក៏​ពិត​មែន ប៉ុន្តែ​មាន​ឥទ្ធិពល​ខ្លាំង​នៅ​ពេល​វា​ដុះ​រួម​គ្នា​ជា​ច្រើន ដែល​បង្ក​ឲ្យ​ក្លាយ​ជា​ពូជ​ស្មៅ​អាក្រក់​ជាង​គេ​បំផុត​លើ​ពិភពលោក​ជា​ច្រើន​ផង​ដែរ។ នៅ​ក្នុង​សៀវភៅ​មគ្គទេសក៍​នេះ យើង​បាន​ព្យាយាម​ប្រមូល​ព័ត៌មាន​ជាក់​ស្តែង​អំពី​ប្រភេទ​ស្មៅ​ចង្រៃ​នៅ​ក្នុង​ស្រែ​ទូទៅ​មួយ​ចំនួន​នៅ​តំបន់​អាស៊ី រួម​ទាំង​ព័ត៌មាន​អំពី​រុក្ខវិទ្យា បរិស្ថាន​វិទ្យា ភាព​ធន់​ទ្រាំ​ទៅ​នឹង​ថ្នាំ​សម្លាប់​ស្មៅ​និង​ការ​កម្ចាត់​តាម​វិធីសាស្រ្ត​ដាំដុះ ជា​លក្ខណៈ​អត្ថបទ​ខ្លីៗ​ដែល​អាច​ផ្តល់​ភាព​ងាយ​ស្រួល​ក្នុង​ការ​ប្រើ​ប្រាស់។​ លើស​ពីនេះ​ទៅ​ទៀត យើង​ក៏​បាន​ដាក់​បញ្ចូល​ជា​រូបភាព​ដើម្បី​ឲ្យ​យល់​កាន់​តែ​ច្បាស់​និង​បាន​ត្រឹម​ត្រូវ​អំពី​អត្តសញ្ញាណ​នៃ​ស្មៅ​ចង្រៃ​នីមួយៗ​ផងដែរ។



ចំណងជើង:
ពូជស្រូវ ផ្ការំដួលប្រាំង
បរិយាយ:

ពូជស្រូវ ”ផ្ការំដួលប្រាំង” ជាពូជស្រូវប្រកាន់រដូវខ្សោយ ដែលបានធ្វើការបញ្ចេញ
និងផ្សព្វផ្សាយជូនកសិករប្រើប្រាស់ដោយ វិទ្យាស្ថានស្រាវជ្រាវនិងអភិវឌ្ឍន៏
កសិកម្មកម្ពុជា នៃក្រសួងកសិកម្ម រុក្ខាប្រមាញ់ និងនេសាទ នាដើមឆ្នាំ២០១៥។ 

ពូជស្រូវ ”
ផ្ការំដួលប្រាំង” ជាពូជស្រូវដែលបានសម្រិតសម្រាំងតាមវិធីសាស្ត្រ
ជម្រើសឯកត្តៈ ពីពូជស្រូវផ្ការំដួលចាប់ពីឆ្នាំ២០០៦ សំដៅឲ្យកសិករមាន
លទ្ធភាពដាំដុះពូជស្រូវដែលមានគុណភាពប្រណីតនិងតម្លៃខ្ពស់ក្នុងរដូវប្រាំង។

 

keyword:
N/A

ពូជស្រូវ ”ផ្ការំដួលប្រាំង” ជាពូជស្រូវប្រកាន់រដូវខ្សោយ ដែលបានធ្វើការបញ្ចេញ
និងផ្សព្វផ្សាយជូនកសិករប្រើប្រាស់ដោយ វិទ្យាស្ថានស្រាវជ្រាវនិងអភិវឌ្ឍន៏
កសិកម្មកម្ពុជា នៃក្រសួងកសិកម្ម រុក្ខាប្រមាញ់ និងនេសាទ នាដើមឆ្នាំ២០១៥។ 

ពូជស្រូវ ”
ផ្ការំដួលប្រាំង” ជាពូជស្រូវដែលបានសម្រិតសម្រាំងតាមវិធីសាស្ត្រ
ជម្រើសឯកត្តៈ ពីពូជស្រូវផ្ការំដួលចាប់ពីឆ្នាំ២០០៦ សំដៅឲ្យកសិករមាន
លទ្ធភាពដាំដុះពូជស្រូវដែលមានគុណភាពប្រណីតនិងតម្លៃខ្ពស់ក្នុងរដូវប្រាំង។

 



ចំណងជើង:
របាយការណ៍សង្ខេបសមិទ្ធផលនៃការស្រាវជ្រាវនិងអភិវឌ្ឍន៍បច្ចេកវិទ្យាកសិកម្មឆ្នាំ២០១៧​ និងទិសដៅសម្រាប់ឆ្នាំ២០១៨
បរិយាយ:
 របាយការណ៍សង្ខេបសមិទ្ធផលនៃការស្រាវជ្រាវនិងអភិវឌ្ឍន៍បច្ចេកវិទ្យាកសិកម្មឆ្នាំ២០១៧​ និងទិសដៅសម្រាប់ឆ្នាំ២០១៨
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 របាយការណ៍សង្ខេបសមិទ្ធផលនៃការស្រាវជ្រាវនិងអភិវឌ្ឍន៍បច្ចេកវិទ្យាកសិកម្មឆ្នាំ២០១៧​ និងទិសដៅសម្រាប់ឆ្នាំ២០១៨


ចំណងជើង:
របាយការណ៍ស្តីពីលទ្ធផលការងាររបស់វិទ្យាស្ថានក្រោមការដឹកនាំក្រុមប្រឹក្សាភិបាលអាណតិ្តទី៥
បរិយាយ:
 
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ចំណងជើង:
ទស្សនាវដ្តីកម្ពុជា Volume 8, Number 1 Jan-Jun 2007
បរិយាយ:
អំបូររុក្ខជាតិ “ផ្លែស្លាបភ្លោះ”

ប្រសិទ្ធិភាពមើមដំឡូងឈើផ្អាប់ និងកន្ទក់លើការលូតលាស់របស់កសិករ នៅខេត្ដពោធិសាត់

ស្លឹកកន្ទុំថេតជួយទ្រទ្រង់ការលូតលាស់របស់ត្រីបានពាក់កណ្ដាលនៃវដ្ដចិញ្ចឹម

ការដាំដុះទ្វេដំណាំដែលមានដំណាំស្រូវវស្សាជាគ្រឹះ នៅប្រទេសកម្ពុជា   

កំពង់សៀម កាល់គែៈ ជាអនុក្រុមថ្មីនៃក្រុមដីកំពង់សៀម សម្រាប់ចំណាត់ថ្នាក់ដីកសិកម្មកម្ពុជា
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អំបូររុក្ខជាតិ “ផ្លែស្លាបភ្លោះ”

ប្រសិទ្ធិភាពមើមដំឡូងឈើផ្អាប់ និងកន្ទក់លើការលូតលាស់របស់កសិករ នៅខេត្ដពោធិសាត់

ស្លឹកកន្ទុំថេតជួយទ្រទ្រង់ការលូតលាស់របស់ត្រីបានពាក់កណ្ដាលនៃវដ្ដចិញ្ចឹម

ការដាំដុះទ្វេដំណាំដែលមានដំណាំស្រូវវស្សាជាគ្រឹះ នៅប្រទេសកម្ពុជា   

កំពង់សៀម កាល់គែៈ ជាអនុក្រុមថ្មីនៃក្រុមដីកំពង់សៀម សម្រាប់ចំណាត់ថ្នាក់ដីកសិកម្មកម្ពុជា
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